Boekel Scientific RapidFISH Slide Hybridizer, 240200, Microscope slide oven (115V/230V)
Boekel Scientific RapidFISH Slide Hybridizer, 240200, Microscope slide oven (115V/230V) Boekel Scientific RapidFISH Slide Hybridizer, 240200, Microscope slide oven (115V/230V) Boekel Scientific RapidFISH Slide Hybridizer, 240200, Microscope slide oven (115V/230V) Boekel Scientific RapidFISH Slide Hybridizer, 240200, Microscope slide oven (115V/230V) Boekel Scientific RapidFISH Slide Hybridizer, 240200, Microscope slide oven (115V/230V)

Boekel Scientific RapidFISH Slide Hybridizer, 240200, Microscope slide oven (115V/230V)

In stock

SKU 240200 & 240200-2
The RapidFISH was designed to simplify Fluorescent In-Situ Hybridization (FISH) procedures. The unit has a self-clamping slide tray design that maintains the required humidity. The control system provides excellent temperature stability and uniformity. Small benchtop design saves space in your lab.
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Boekel Scientific RapidFISH Slide Hybridizer, 240200, Microscope slide oven (115V/230V)
Boekel Scientific RapidFISH Slide Hybridizer, 240200, Microscope slide oven (115V/230V)

In stock

$132.46

Summary

    The RapidFISH was designed to simplify Fluorescent In-Situ Hybridization (FISH) procedures for FFPE (formalin fixed paraffin embedded tissue) samples, cell suspensions, and other tissue preparation procedures. Temperature control and humidity are essential for FISH probes to bind to RNA and DNA. The RapidFISH has a self-clamping slide tray design that maintains the required humidity. Microscope slides in the RapidFISH do not require coverslips to be glued because the removable tray of the slide incubator traps moisture in the system. Elimination of this step decreases processing time and ensures that the specimens stay hydrated during processing. The system has an easy to use control system for controlling temperature, time and controlled temperature steps. The device has a small footprint and still maintains excellent temperature stability and uniformity because of its dual heater convection system. FISH testing is used worldwide to identify biological markers in the form of DNA/RNA. 

    • Self-clamping slide tray, ideal for hybridization
    • No coverslip glue necessary
    • Dual heater convection system for excellent temperature control
    • Process up to twenty (20) 3”x 1” glass microscope slides
    • Includes slide tray and metal slide holder
    • Easy to use control system
    • Includes RapidFISH Slide Hybridizer, Slide Tray, Slide Holder

    Application Highlights
    Histology, Pathology, FFPE sample storage, Cancer Research, Cancer Diagnosis, Genetic Research, Genetic Diagnosis  

    Application Overview  
    Molecular diagnostics is the fastest growing segment of the diagnostics market.  Flourescent In Situ Hybridization or FISH analysis comprises 700 Million of this 5 Billion dollar market.  The FISH market is projected to grow at 11% year over year for the next five years.  400 to 500 million of this market is generated in the United States.  FISH testing is used to identify biological markers in the form of DNA/ RNA.  It is well-known method for genetic mapping and gene expression profiling.  This technique uses fluorescent dye-labeled probes and a nucleotide sequence that is mapped to a chromosome or intra-nuclear DNA to generate a fluorescence signal which is visualized in situ by microscopy.  It is performed on fresh preparations such as cytospins(cell suspensions) and touch preps or formalin-fixed, paraffin-embedded (FFPE) tissues may be used.   It is used for research and clinical diagnostics in the areas of:

    • Prenatal diagnosis
    • Cancer diagnosis
    • Molecular cytogenetic of birth defects and mental retardation
    • Chromosomal abnormalities
    • Clinical genetics
    • Neuroscience
    • Reproductive medicine
    • Toxicology  
    • Microbial ecology
    • Evolutionary biology
    • Comparative genomics
    • Cellular genomics
    • Differentiation between benign and malignant tumors

    The majority of this 700 million market is for reagents including FDA approved tests, companion diagnostics, research use only probes, custom probes and support reagents and supplies.  There is a need for hybridization equipment to perform these labor intensive tests.  To understand the need for hybridization equipment the process requirements must clearly be defined.

     The equipment necessary is as follows:

    • pH meter
    • Balance
    • Stir plate
    • Micro-centrifuge
    • Refrigerator
    • Freezer

    Tissue preparation will require:

    • Microtome
    • Water bath
    • Oven

    Performing the FISH procedure will require the following equipment and materials in addition to reagents:

    • Slide dryer
    • Water baths
    • Hybridization Oven
    • Coplin jars
    • Coverslips
    • Staining dishes
    • Rubber cement
    • Fluorescent microscopes DAPI filter (blue), FITC filter (green), and a Texas red filter capable of photography


    The FISH Process - Simplified
    Sample prep – depariffinization, dehydrate, air dry, pretreatment(95C @ 30 min, Coplin Jar), protease treatment(37°C 10 – 20 min, Coplin jar), Dehydrate, Air Dry, Mark, apply fish probe,   
    Place Cover glass with cement
    Denature -  5 minutes @ 75°C (45 – 50°C for metaphase spread + additional step to denature probe) Hybridization - 37°C 16hr – 72hr
    Labeling Process
    Direct Labeling – label is bound to the probe and it is less sensitive
    Or    -
    Indirect Labeling – Additional step before detection, probe detected using antibodies, results in amplified single
    Storage – 4 – 8°C for up to two months, in dark area – light sensitive  

    Crucial Process Steps
    Two steps in the FISH process require equipment for heating the slides; the denaturing step and the hybridization.  These steps are similar for most FFPE FISH procedures.  
    Denature: Denaturing the probes and double stranded DNA
    Average Time: 5 Minutes
    Standard Temperature: 75°C
    Hybridization:  Hybridization of the probes to the target nucleic acids
    Time Range: 16 – 72 Hours
    Standard Temperature: 37°C
    Developed slides must be stored at 4 – 8°C until they are evaluated under a microscope and photographed.  They can be store for months at these temperatures.  

    List of Specific FISH FDA approved Tests

    Herceptin

    (trastuzumab)

    BLA 103792

    INFORM HER-2/NEU

    P940004S001

    Ventana Medical Systems, Inc.

    The Inform Her-2/Neu gene detection system is a fluorescence in situ hybridization (FISH) DNA probe assay that determines the qualitative presence of Her-2/Neu gene amplification on formalin-fixed, paraffin embedded human breast tissue as an aid to stratify breast cancer patients according to risk for recurrence or disease-related death. It is indicated for use as an adjunct to existing clinical and pathologic information currently used as prognostic indicators in the risk stratification of breast cancer in patients who have had a priori invasive, localized breast carcinoma and who are lymph node-negative.

    Herceptin

    (trastuzumab)

    BLA 103792

    PATHVYSION HER-2 DNA Probe Kit

    P980024S001-S012

    Abbott Molecular Inc.

    The PathVysion HER-2 DNA Probe Kit (PathVysion Kit) is designed to detect amplification of the HER-2/neu gene via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded human breast cancer tissue specimens. Results from the PathVysion Kit are intended for use as an adjunct to existing clinical and pathologic information currently used as prognostic factors in stage II, node-positive breast cancer patients. The PathVysion Kit is further indicated as an aid to predict disease-free and overall survival in patients with stage II, node positive breast cancer treated with adjuvant cyclophosphamide, doxorubicin, and 5-fluorouracil (CAF) chemotherapy. The Pathvysion Kit is indicated as an aid in the assessment of patients for whom herceptin (trastuzumab) treatment is being considered (see herceptin package insert).

    Herceptin

    (trastuzumab)

    BLA 103792

    PATHWAY ANTI-HER-2/NEU (4B5) Rabbit Monoclonal Primary Antibody

    P990081S001-S028

    Ventana Medical Systems, Inc.

    Ventana Medical Systems’ PATHWAY Her2 (clone CB11) is a mouse monoclonal antibody intended for laboratory use for the semi-quantitative detection of c-erbB-2 antigen in sections of formalin-fixed, paraffin-embedded normal and neoplastic tissue on a Ventana automated immunohistochemistry slide staining device. 

    Disease

    Trade Name

    Manufacturer

    Submission

    Acute Myeloid Leukemia

    Vysis D7S486/CEP 7 FISH Probe Kit

    Abbott Molecular Inc.

    K131508

     

    Vysis EGR1 FISH Probe Kit

    Abbott Molecular Inc.

    K123951, K091960

    B-cell chronic lymphocytic leukemia

    Vysis CLL FISH Probe Kit

    Vysis

    K100015

    Prosigna Breast Cancer Prognostic Gene Signature Assay

    Nanostring Technologies

     

    K130010

    MammaPrint

    Agendia BV

     

    K101454K081092,K080252K070675,K062694

    INFORM HER2 Dual ISH DNA Probe Cocktail

    Ventana Medical Systems, Inc.

     

    P100027

    HER2 CISH pharmDxTM Kit

    Dako Denmark A/S

     

    P100024

    GeneSearch Breast Lymph Node (BLN) Test Kit

    Veridex, LLC.

     

    P060017 S001-S004

    Dako TOP2A FISH PharmDx Kit

    Dako Denmark A/S

     

    P050045 S001-S004

    DakoCytomation Her2 FISH pharmDx™ Kit

    DakoCytomation Denmark A/S

     

    P040005

    BD Max MRSA Assay

    BD Diagnostics (GeneOhm Sciences Canada, Inc.)

     

    K120138

    Staphylococcus QuickFISH BC

    AdvanDx, Inc.

     

    K113371

    MRSA/SA ELITe MGB

    EliTechGroup Epoch Biosciences.

     

    K112937

    Verigene Staphylococcus Blood Culture (BC-S) Nucleic Acid Test

    Nanosphere, Inc.

     

    K112424

    NucliSENS EasyQ MRSA Assay

    bioMerieux, Inc.

     

    K102740

    Xpert MRSA/SA Blood Culture Assay

    Cepheid

     

    K101879K082140

    Xpert MRSA/SA Nasal Complete Assay

    Cepheid

     

    K100822

    LightCycler MRSA Advanced Test

    Roche Molecular Systems, Inc

     

    K091409

    BD GeneOhm MRSA ACP Assay

    BD Diagnostics (GeneOhm Sciences Canada, Inc.)

     

    K093346

    S. aureus and/or other Staphylococcus species PNA FISH Culture

    AdvanDx, Inc.

     

    K092166

    Xpert MRSA/SA SSTI Assay

    Cepheid

     

    K080837

    BD GeneOhM StaphSR Assay

    BD Diagnostics (GeneOhm Sciences Canada, Inc.)

     

    K071026

    S. aureus PNA FISH

    AdvanDx, Inc.

     

    K091827K060099

    Xpert MRSA

    Cepheid

     

    K070462

    IDI-MRSA Assay

    Infectio Diagnostic, Inc.

     

    K042357K033415

    Microorganism

    Trade Name

    Manufacturer

    Submission

    Adenovirus

    Adenovirus R-gene US

    Argene SA

    K121942

     

    ProAdeno+ Assay

    Gen-Probe Prodesse, Inc.

    K102952

    Bacillus Anthracis

    JBAIDS Anthrax Detection System

    Idaho Technology, Inc.

    K131930K071188,K051713

    Candida tropicalis / 

    Candida albicans / 

    Candida parapsilosis / 

    Candida glabrata / 

    Candida krusei  

    Affirm VPIII Microbial Identification Test

    Microprobe Corp.

    K931151K931374

     

    Yeast Traffic Light PNA FISH

    AdvanDx, Inc.

    K093024K080719

     

    C. albicans / C. glabrata PNA FISH

    AdvanDx, Inc.

    K092784

     

    C. albicans PNA FISH

    AdvanDx, Inc.

    K092655K062461

     

    C. Albicans screen

    Carr-Scarborough Microbiologicals, Inc.

    K891476

    Entercoccus

    IMDx Van R for Abbott m2000

    Intelligent Medical Devices, Inc.

    K123753

     

    BD GeneOhm VanR Assay

    BD Diagnostics (GeneOhm Sciences, Inc.)

    K102416

     

    Xpert vanA Assay

    Cepheid

    K092953

     

    E. faecalis/OE PNA

    AdvanDx, Inc.

    K083074

     

    GBS PNA FISH

    AdvanDx, Inc.

    K082612

     

    E. faecalis PNA FISH culture identification kit

    AdvanDx, Inc.

    K063127

    Enterovirus

    NucliSens EasyQ Enterovirus vl.1 Assay

    bioMérieux, Inc.

    K093383K063261

    Escherichia coli / 

    Klebsiella pneumoniae / 

    Pseudomonas aeroginosa

    GNR Traffic Light PNA FISH

    AdvanDx, Inc.

    K101558

     

    E. coli/P. aeruginosa PNA FISH

    AdvanDx, Inc.

    K092236K081309

     

    EK/P aeruginosa PNA FISH

    AdvanDx, Inc.

    K092393K081433

     

    E. coli PNA FISH

    AdvanDx, Inc.

    K082068

    Staphylococcus

    BD Max MRSA Assay

    BD Diagnostics (GeneOhm Sciences Canada, Inc.)

    K120138

     

    Staphylococcus QuickFISH BC

    AdvanDx, Inc.

    K113371

    Types of FISH Hybridization Equipment
    Manual:  The operator is responsible for all liquid additions to the slide to properly expose the assay.  The equipment manages all temperature changes and timers.  These units accommodate 10 – 30 slides
    Automatic: Controls the liquid additions and heating of slides so all that the operator has to do is to load the samples.  Manual systems are much lower cost and accommodate 10 – 64 slides.