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Slide Moat™

Slide Moat™

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$81.81

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Quick Overview

240000 & 240000-2
Designed for use with FISH protocols, provides excellent temperature uniformity and stability. 30 slide capacity

Price as configured: $0.00

Details

  • PID temperature controller for easy temperature selection
  • Tempered glass lid which seals with integral gasket on heating base
  • Capacity for 30 standard microscope slides
  • Optional light shield for photosensitive reagents is available
  • Excellent temperature uniformity and stability
  • 2 year warranty

Applications

  • In situ hybridization
  • All single temperature microscope slide incubation procedures
  • Slide warming and drying
  • Immuno-histochemistry
  • FISH
  • Histology, Hematology

 

FISH  Overview

Molecular diagnostics is the fastest growing segment of the diagnostics market.  Flourescent In Situ Hybridization or FISH analysis comprises 700 Million of this 5 Billion dollar market.  The FISH market is projected to grow at 11% year over year for the next five years.  400 to 500 million of this market is generated in the United States.  FISH testing is used to identify biological markers in the form of DNA/ RNA.  It is well-known method for genetic mapping and gene expression profiling.  This technique uses fluorescent dye-labeled probes and a nucleotide sequence that is mapped to a chromosome or intra-nuclear DNA to generate a fluorescence signal which is visualized in situ by microscopy.  It is performed on fresh preparations such as cytospins(cell suspensions) and touch preps or formalin-fixed, paraffin-embedded (FFPE) tissues may be used.   It is used for research and clinical diagnostics in the areas of:

• Prenatal diagnosis

• Cancer diagnosis

• Molecular cytogenetic of birth defects and mental retardation

• Chromosomal abnormalities

• Clinical genetics

• Neuroscience

• Reproductive medicine

• Toxicology 

• Microbial ecology

• Evolutionary biology

• Comparative genomics

• Cellular genomics

• Differentiation between benign and malignant tumors

The majority of this market is for reagents including FDA approved tests, companion diagnostics, research use only probes, custom probes and support reagents and supplies.  There is a need for hybridization equipment to perform these labor intensive tests.  To understand the need for hybridization equipment the process requirements must clearly be defined.

The equipment necessary is as follows:

-              pH meter

-              Balance

-              Stir plate

-              Micro-centrifuge

-              Refrigerator

-              Freezer

Tissue preparation will require:

-              Microtome

-              Water bath

-              Oven

Performing the FISH procedure will require the following equipment and materials in addition to reagents:

-              Slide dryer

-              Water baths

-              Hybridization Oven

-              Coplin jars

-              Coverslips

-              Staining dishes

-              Rubber cement (Optional: depends on equipment used)

-              Fluorescent microscopes DAPI filter (blue), FITC filter (green), and a Texas red filter capable of photography

 

The FISH Process - Simplified

Sample prep – depariffinization, dehydrate, air dry, pretreatment(95C @ 30 min, Coplin Jar), protease treatment(37°C 10 – 20 min, Coplin jar), Dehydrate, Air Dry, Mark, apply fish probe,  

Place Cover glass with cement

Denature -  5 minutes @ 75°C (45 – 50°C for metaphase spread + additional step to denature probe) Hybridization - 37°C 16hr – 72hr

Labeling Process

Direct Labeling – label is bound to the probe and it is less sensitive

-              Or    -

Indirect Labeling – Additional step before detection, probe detected using antibodies, results in amplified single

Storage – 4 – 8°C for up to two months, in dark area – light sensitive 

 

Crucial Process Steps

Two steps in the FISH process require equipment for heating the slides; the denaturing step and the hybridization.  These steps are similar for most FFPE FISH procedures. 

Denature: Denaturing the probes and double stranded DNA

Average Time: 5 Minutes

Standard Temperature: 75°C

Hybridization:  Hybridization of the probes to the target nucleic acids

Time Range: 16 – 72 Hours

Standard Temperature: 37°C

Developed slides must be stored at 4 – 8°C until they are evaluated under a microscope and photographed.  They can be store for months at these temperatures. 

 

List of Specific FISH Tests

There are thousands of FISH probes used for research supplied by dozens of companies.  These do not have FDA approval.  The FDA approved test that use the FISH procedure are listed below. 

 

Herceptin

(trastuzumab)

BLA 103792

INFORM HER-2/NEU

P940004S001

Ventana Medical Systems, Inc.

The Inform Her-2/Neu gene detection system is a fluorescence in situ hybridization (FISH) DNA probe assay that determines the qualitative presence of Her-2/Neu gene amplification on formalin-fixed, paraffin embedded human breast tissue as an aid to stratify breast cancer patients according to risk for recurrence or disease-related death. It is indicated for use as an adjunct to existing clinical and pathologic information currently used as prognostic indicators in the risk stratification of breast cancer in patients who have had a priori invasive, localized breast carcinoma and who are lymph node-negative.

Herceptin

(trastuzumab)

BLA 103792

PATHVYSION HER-2 DNA Probe Kit

P980024S001-S012

Abbott Molecular Inc.

The PathVysion HER-2 DNA Probe Kit (PathVysion Kit) is designed to detect amplification of the HER-2/neu gene via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded human breast cancer tissue specimens. Results from the PathVysion Kit are intended for use as an adjunct to existing clinical and pathologic information currently used as prognostic factors in stage II, node-positive breast cancer patients. The PathVysion Kit is further indicated as an aid to predict disease-free and overall survival in patients with stage II, node positive breast cancer treated with adjuvant cyclophosphamide, doxorubicin, and 5-fluorouracil (CAF) chemotherapy. The Pathvysion Kit is indicated as an aid in the assessment of patients for whom herceptin (trastuzumab) treatment is being considered (see herceptin package insert).

Herceptin

(trastuzumab)

BLA 103792

PATHWAY ANTI-HER-2/NEU (4B5) Rabbit Monoclonal Primary Antibody

P990081S001-S028

Ventana Medical Systems, Inc.

Ventana Medical Systems’ PATHWAY Her2 (clone CB11) is a mouse monoclonal antibody intended for laboratory use for the semi-quantitative detection of c-erbB-2 antigen in sections of formalin-fixed, paraffin-embedded normal and neoplastic tissue on a Ventana automated immunohistochemistry slide staining device.

 

Disease

Trade Name

Manufacturer

Submission

Acute Myeloid Leukemia

Vysis D7S486/CEP 7 FISH Probe Kit

Abbott Molecular Inc.

K131508

 

Vysis EGR1 FISH Probe Kit

Abbott Molecular Inc.

K123951, K091960

B-cell chronic lymphocytic leukemia

Vysis CLL FISH Probe Kit

Vysis

K100015

Prosigna Breast Cancer Prognostic Gene Signature Assay

Nanostring Technologies

 

K130010

MammaPrint

Agendia BV

 

K101454K081092,K080252K070675,K062694

INFORM HER2 Dual ISH DNA Probe Cocktail

Ventana Medical Systems, Inc.

 

P100027

HER2 CISH pharmDxTM Kit

Dako Denmark A/S

 

P100024

GeneSearch Breast Lymph Node (BLN) Test Kit

Veridex, LLC.

 

P060017 S001-S004

Dako TOP2A FISH PharmDx Kit

Dako Denmark A/S

 

P050045 S001-S004

DakoCytomation Her2 FISH pharmDx™ Kit

DakoCytomation Denmark A/S

 

P040005

BD Max MRSA Assay

BD Diagnostics (GeneOhm Sciences Canada, Inc.)

 

K120138

Staphylococcus QuickFISH BC

AdvanDx, Inc.

 

K113371

MRSA/SA ELITe MGB

EliTechGroup Epoch Biosciences.

 

K112937

Verigene Staphylococcus Blood Culture (BC-S) Nucleic Acid Test

Nanosphere, Inc.

 

K112424

NucliSENS EasyQ MRSA Assay

bioMerieux, Inc.

 

K102740

Xpert MRSA/SA Blood Culture Assay

Cepheid

 

K101879K082140

Xpert MRSA/SA Nasal Complete Assay

Cepheid

 

K100822

LightCycler MRSA Advanced Test

Roche Molecular Systems, Inc

 

K091409

BD GeneOhm MRSA ACP Assay

BD Diagnostics (GeneOhm Sciences Canada, Inc.)

 

K093346

S. aureus and/or other Staphylococcus species PNA FISH Culture

AdvanDx, Inc.

 

K092166

Xpert MRSA/SA SSTI Assay

Cepheid

 

K080837

BD GeneOhM StaphSR Assay

BD Diagnostics (GeneOhm Sciences Canada, Inc.)

 

K071026

S. aureus PNA FISH

AdvanDx, Inc.

 

K091827K060099

Xpert MRSA

Cepheid

 

K070462

IDI-MRSA Assay

Infectio Diagnostic, Inc.

 

K042357K033415

 

 

 

 

Microorganism

Trade Name

Manufacturer

Submission

Adenovirus

Adenovirus R-gene US

Argene SA

K121942

 

ProAdeno+ Assay

Gen-Probe Prodesse, Inc.

K102952

Bacillus Anthracis

JBAIDS Anthrax Detection System

Idaho Technology, Inc.

K131930K071188,K051713

Candida tropicalis / 

Candida albicans / 

Candida parapsilosis / 

Candida glabrata / 

Candida krusei  

Affirm VPIII Microbial Identification Test

Microprobe Corp.

K931151K931374

 

Yeast Traffic Light PNA FISH

AdvanDx, Inc.

K093024K080719

 

C. albicans / C. glabrata PNA FISH

AdvanDx, Inc.

K092784

 

C. albicans PNA FISH

AdvanDx, Inc.

K092655K062461

 

C. Albicans screen

Carr-Scarborough Microbiologicals, Inc.

K891476

  

Entercoccus

IMDx Van R for Abbott m2000

Intelligent Medical Devices, Inc.

K123753

 

BD GeneOhm VanR Assay

BD Diagnostics (GeneOhm Sciences, Inc.)

K102416

 

Xpert vanA Assay

Cepheid

K092953

 

E. faecalis/OE PNA

AdvanDx, Inc.

K083074

 

GBS PNA FISH

AdvanDx, Inc.

K082612

 

E. faecalis PNA FISH culture identification kit

AdvanDx, Inc.

K063127

Enterovirus

NucliSens EasyQ Enterovirus vl.1 Assay

bioMérieux, Inc.

K093383K063261

Escherichia coli / 

Klebsiella pneumoniae / 

Pseudomonas aeroginosa

GNR Traffic Light PNA FISH

AdvanDx, Inc.

K101558

 

E. coli/P. aeruginosa PNA FISH

AdvanDx, Inc.

K092236K081309

 

EK/P aeruginosa PNA FISH

AdvanDx, Inc.

K092393K081433

 

E. coli PNA FISH

AdvanDx, Inc.

K082068

Staphylococcus

BD Max MRSA Assay

BD Diagnostics (GeneOhm Sciences Canada, Inc.)

K120138

 

Staphylococcus QuickFISH BC

AdvanDx, Inc.

K113371

 

Citations

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Langie, SA;Cameron, KM;Waldron, KJ;Fletcher, KP;von Zglinicki, T;Mathers, JC;. "Measuring DNA repair incision activity of mouse tissue extracts towards singlet oxygen-induced DNA damage: a comet-based in vitro repair assay." Mutagenesis. 26 (2011): 461-71. Web. <http://mutage.oxfordjournals.org/content/early/2011/02/25/mutage.ger005.full>

Ghazi, A;Ashoori, A;Hanley, PJ;Brawley, VS;Shaffer, DR;Kew, Y;Powell, SZ;Grossman, R;Grada, Z;Scheurer, ME;Hegde, M;Leen, AM;Bollard, CM;Rooney, CM;Heslop, HE;Gottschalk, S;Ahmed, N;. "Generation of polyclonal CMV-specific T cells for the adoptive immunotherapy of glioblastoma." J. Immunother.. 35 (2012): 159-68. Web. <http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280423/>

Whitmore, RG;Krejza, J;Kapoor, GS;Huse, J;Woo, JH;Bloom, S;Lopinto, J;Wolf, RL;Judy, K;Rosenfeld, MR;Biegel, JA;Melhem, ER;O'Rourke, DM;. "Prediction of oligodendroglial tumor subtype and grade using perfusion weighted magnetic resonance imaging." J. Neurosurg.. 107 (2007): 600-9. Web. <http://thejns.org/doi/full/10.3171/JNS-07/09/0600>

Sawyer, IA;Shevtsov, SP;Dundr, M;. "Spectral imaging to visualize higher-order genomic organization." Nucleus. (2016): 1-14. Web. <http://www.tandfonline.com/doi/abs/10.1080/19491034.2016.1187344>

Willmore-Payne, C;Holden, J;Turner, KC;Proia, A;Layfield, LJ;. "Translocations and amplifications of chromosome 12 in liposarcoma demonstrated by the LSI CHOP breakapart rearrangement probe."Arch. Pathol. Lab. Med.. 132 (2008): 952-7. Web. <http://www.archivesofpathology.org/doi/full/10.1043/1543-2165(2008)132[952:TAAOCI]2.0.CO;2>

Mazor, R;Korenstein-Ilan, A;Barbul, A;Eshet, Y;Shahadi, A;Jerby, E;Korenstein, R;. "Increased levels of numerical chromosome aberrations after in vitro exposure of human peripheral blood lymphocytes to radiofrequency electromagnetic fields for 72 hours." Radiat. Res.. 169 (2008): 28-37. Web. <http://www.eng.tau.ac.il/~jerby/70.pdf>

Korenstein-Ilan, A;Barbul, A;Hasin, P;Eliran, A;Gover, A;Korenstein, R;. "Terahertz radiation increases genomic instability in human lymphocytes." Radiat. Res.. 170 (2008): 224-34. Web. <http://paloma.eng.tau.ac.il/~gover/120.pdf>

Bowen, KB;Reimers, AP;Luman, S;Kronz, JD;Fyffe, WE;Oxford, JT;. "Immunohistochemical localization of collagen type XI alpha1 and alpha2 chains in human colon tissue." J. Histochem. Cytochem..56 (2008): 275-83. Web. <http://jhc.sagepub.com/content/56/3/275.full>

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Sato, Y;Shimonohara, N;Hanaki, K;Goto, M;Yamakawa, Y;Horiuchi, M;Takahashi, H;Sata, T;Nakajima, N;. "ImmunoAT method: An initial assessment for the detection of abnormal isoforms of prion protein in formalin-fixed and paraffin-embedded tissues." J. Virol. Methods. 165 (2010): 261-7. Web. <http://www.sciencedirect.com/science/article/pii/S0166093410000339>

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Kim, EK;Kim, S;. "ALK gene copy number gain and immunohistochemical expression status using three antibodies in neuroblastoma." Pediatr. Dev. Pathol.. (2016): . Web. <http://www.pedpath.org/doi/abs/10.2350/15-12-1751-OA.1>

Corao, DA;Biegel, JA;Coffin, CM;Barr, FG;Wainwright, LM;Ernst, LM;Choi, JK;Zhang, PJ;Pawel, BR;. "ALK expression in rhabdomyosarcomas: correlation with histologic subtype and fusion status.."Pediatr. Dev. Pathol.. 12 (2009): 275-83. Web. <http://www.pedpath.org/doi/abs/10.2350/08-03-0434.1>

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Catez, F;Rousseau, A;Labetoulle, M;Lomonte, P;. "Detection of the genome and transcripts of a persistent DNA virus in neuronal tissues by fluorescent in situ hybridization combined with immunostaining." J Vis Exp. (2014): e51091. Web. <http://www.jove.com/video/51091/detection-genome-transcripts-persistent-dna-virus-neuronal-tissues>

Specs

Catalog Number: 240000, 240000-2
Temperature Range: Ambient +5°C to 100°C
Accuracy: ± 0.2°C
Heat Up Rate: Ambient to 65°C in 20 minutes
Slide Capacity: 30- 1" x 3" (three rows of ten)
Clearance between lid and slide pedestal: 1.0cm (0.48")
Overall Dimensions W x D x H: 16" x 133⁄4" x 53⁄4"
Overall Dimensions W x D x H: 40.6cm x 34.9cm x 14.6cm
Shipping Weight: 10.4kg (23lbs.)
Electrical: 240000 115VAC 60Hz(UL/CSA)
240000-2 230VAC 50Hz; CE Marked*
Note: We strongly recommend using covered and sealed slides for higher temperatures (>37°C) or prolonged periods (> 1 hour) incubations.
*230V units are supplied with continental European plug.

Attachments

View FileHistology Products Brochure    Size: (1.8 MB)
View FileSlide Moat Manual    Size: (109.67 KB)
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